[1]韩汶雨,刘建星,徐钰童,等.东北对开蕨孢子离体快繁技术研究[J].现代农业研究,2020,(6):38-39.
 HANWenyu,LIU Jianxing,XU Yutong,et al.Study on in vitro rapid propagation of the spore of Potentilla anserina from Northeast China[J].Modern Agricultural Research,2020,(6):38-39.
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东北对开蕨孢子离体快繁技术研究
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《现代农业研究》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年6期
页码:
38-39
栏目:
作物科学
出版日期:
2020-06-01

文章信息/Info

Title:
Study on in vitro rapid propagation of the spore of Potentilla anserina from Northeast China
文章编号:
2096-1073(2020)06-0038-39
作者:
韩汶雨刘建星徐钰童陈浩宇于海阔刘畅李余先建德锋*
吉林农业科技学院中药学院
Author(s):
HANWenyu LIU Jianxing XU Yutong CHEN Haoyu YU Haikuo LIU Chang LI Yuxian JIAN Defeng*
College of Traditional Chinese Medicine, Jilin Agricultural Science and Technology University
关键词:
孢子东北对开蕨原叶体增殖孢子体诱导
Keywords:
spore potentilla anserina northeasterly protoplast proliferation sporophytic induction
分类号:
S682.35
文献标志码:
A
摘要:
该实验以东北对开蕨的孢子为外植体进行离体培养试验,得出了东北对开蕨孢子离体快繁 的途径:孢子试管培养初步得出原叶体,原叶体通过扩繁数量增多,然后将这些原叶体进行试管外诱 导得到孢子体,培养孢子体即可得到东北对开蕨的植株。另通过配方比较得出:试管内采用MS 培养 基增殖效果最好,试管外培养过程中定期喷施0.1% KH2PO4 诱导剂效果最好。
Abstract:
The experiment to the northeast divided fern spores of explant in vitro culture experiment was car? ried out, it is concluded that the northeast divided fern spores in vitro rapid propagation path: preliminary draw prothallus spores in vitro, prothallus through propagation quantity increased, and then will be in vitro in? duced to get these prothallus sporophyte, cultivate sporophyte obtains the northeast divided fern plants. In ad? dition, formula comparison showed that MS medium had the best effect of proliferation in vitro and 0.1% KH2PO4 inducer was sprayed regularly in vitro.

参考文献/References:

[1] 张月雅,刘明磊,颜小梅,等.4 种蕨类植物耐热性测定[J].吉林 农业大学学报,2015,37(03):313-316. [2] 樊清伟,邓志刚,张冬昱.长白山蕨类植物资源的研究进展[J]. 通化师范学院学报,2010,31(02):34-36+76. [3] 建德锋,辛立红,马爽.金银忍冬腋芽离体培养技术研究[J].吉 林农业科技学院学报,2014,23(02):11-13+16. [4] 顾德峰,李东升,王雷,等. 东亚对开蕨离体快繁的研究[J].园 艺学报,2008,35(9):1373-1376. [5] 王阳,董然,顾德峰,等.东北对开蕨孢子体诱导技术的研究 [J].北方园艺,2014(19):93-98. [6] 刘晓龙,宋畅,白旭然,等.铁线蕨组培快繁技术研究[J].吉林农 业科技学院学报,2013,22(01):14-15+21.

备注/Memo

备注/Memo:
基金项目:吉林省大学生创新创业项目([2019]002)。 作者简介:韩汶雨(2000-),男,河南省洛阳市人,研究方向:中药栽培。 通讯作者:建德锋(1976-),男,河南省灵宝市人,副教授,硕士,研究方向:植物繁育。
更新日期/Last Update: 1900-01-01