[1]王欢.肠道病毒71 VP1重组蛋白的纯化与鉴定[J].现代农业研究,2017,(11):53-54.
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肠道病毒71 VP1重组蛋白的纯化与鉴定
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《现代农业研究》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年11期
页码:
53-54
栏目:
经验推广
出版日期:
2017-11-25

文章信息/Info

作者:
王欢
吉林农业科技学院生物工程学院
关键词:
肠道病毒71VP1重组蛋白纯化
Keywords:
EV71 VP1 Recombination protein Purification
摘要:
近年来,肠道病毒71 引发的手足口病发病率在我国呈上升趋势,严重危害儿童健康。本研究利用已有的重组原核表达 载体pET32a-VP1,在大肠杆菌BL21 内诱导其表达VP1 融合蛋白,使用镍柱亲和层析纯化重组蛋白,SDS-PAGE 结果表明重组 蛋白表达且纯化浓度较高,可用于今后的EV71 抗原表位筛选与抗EV71 病毒疫苗开发研究。
Abstract:
Recently, Hand-foot-mouth disease (HFMD) caused by Enterovirus 71 (EV 71) outbreak widely in China, which endanger children’s health. In this study, pET32a-VP1 was expressed in E.coli BL21 with the inducement of IPTG. The recombination protein was purified by Ni2+-NTA and indentified by SDS-PAGE, the result of which showed high purity of the recombination protein of VP1.It could provide the bases for research of EV71 epitope screening and new anti-EV71 vaccines.

参考文献/References:

[1] 金奇.医学分子病毒学[M].北京:科学出版社,2000:606-613. [2] Chumakov M, Voroshilova M, Shindarov L, et al. Enterovirus 71 isolated from cases of epidemic poliomyelitis- like disease in bulgaria[J]. Arch Virol, 1979, 60(3/4): 329-340. [3] Blomberg J, Lycke E, Ahlfors K, Johnsson T, Wolontis S, von Zeipel G., Letter: New enterovirus type associated with epidemic of aseptic meningitis and-or hand, foot, and mouth disease[J]. Lancet, 1974,2, 112. [4] Nagy, G., Takatsy, S., Kukan, E., Mihaly, I., Domok, I., 1982. Virological diagnosis of enterovirus type 71 infections: experiences gained during an epidemic of acute CNS diseases in Hungary in 1978[J]. Arch. Virol. 71, 217-227. [5] AbuBakar, S., Chee, H.Y., Al- Kobaisi, M.F., Xiaoshan, J., Chua, K. B., Lam, S.K., 1999. Identification of enterovirus 71 isolates from an outbreak of hand, foot and mouth disease (HFMD) with fatal cases of encephalomyelitis in Malaysia[J]. Virus Res. 61, 1-9. [6] Lee, M.S., Lin, T.Y., Chiang, P.S., Li, W.C., Luo, S.T., Tsao, K.C., Liou, G.Y., Huang, M.L., Hsia, S.H., Huang, Y.C., Chang, S.C., 2008. An investigation of epidemic enterovirus 71 infection in Taiwan, 2008: clinical, virologic, and serologic features. Pediatr[J]. Infect Dis J. 29, 1030- 1034. [7] Mao LX, Wu B, Bao WX, et al. Epidemiology of hand, foot, and mouth disease and genotype characterization of enterovirus 71 in Jiangsu, China [J]. J Clin Virol, 2010, 49(2): 100-104. [8] Podin Y, Gias EL, Ong F, et al. Sentinel surveillance for human enterovirus71 in sarawak, malaysia: lessons from the first 7 years[J]. BMC Public Health, 2006, 6: 180. [9] Oberste MS, Maher K, Kilpatrick DR, et al. Molecular evolution of the human enteroviruses: correlation of serotype with VP1 sequence and application to Picornavirus classification[J].J Virol, 1999, 73(3): 1941- 1948.

备注/Memo

备注/Memo:
*基金项目:吉林省教育厅“十二五”科学技术研究项目(项目编号:吉教科合字[2015]第381号) 作者简介:王欢(1981-),女,讲师,研究方向:重组亚单位疫苗制备学及重组亚单位疫苗制备。
更新日期/Last Update: 2017-11-25