[1]乐贞* 陈旭明 姚水洪 冯敬骞 朱晓萍 陈敏.苏木水提物抗蛇毒磷脂酶A2活性研究[J].现代农业研究,2018,(12):23-26.
　Le Zhen * Chen Xuming Yao Shuihong Feng Jingqian Zhu Xiaoping Chen Min.Study on the water extract of Sappa Lignum against the activity of phospholipase A2 from Snake Venom[J].Modern Agricultural Research,2018,(12):23-26.
点击复制

苏木水提物抗蛇毒磷脂酶A2活性研究
分享到：

《现代农业研究》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年12期
页码:
23-26
栏目:
种植与养殖
出版日期:
2018-12-10

文章信息/Info

Title:
Study on the water extract of Sappa Lignum against the activity of phospholipase A2 from Snake Venom
作者:
乐贞* 陈旭明 姚水洪 冯敬骞 朱晓萍 陈敏
衢州职业技术学院医学院
Author(s):
Le Zhen * Chen Xuming Yao Shuihong Feng Jingqian Zhu Xiaoping Chen Min
QuZhou College of Technology ZheJiang
关键词:
苏木; 五步蛇毒; 抗蛇毒活性
Keywords:
sappan lignum;  agkistrodon acutus venom;  antivenom activity
摘要:
中药苏木(Sappan Lignum)来源于植物苏木(Caesalpinia sappan L.)的干燥心材，具有止血 抗炎等功效。采用琼脂糖平板法研究不同浓度的苏木水提物（0.03g/ml，0.06g/ml，0.09g/ml， 0.12g/ml，0.15g/ml）对蛇毒sPLA2水解活性的抑制作用，测量透明圈和孔的直径，根据酶活标准曲 线计算其抑制率。结果表明0.15g/ml 苏木水提物对五步蛇毒sPLA2水解活性有抑制作用。
Abstract:
The traditional Chinese medicine Sappan Lignum is derived from the dry heartwood of the plant Caesalpinia sappan L. It has the effect of hemostasis and anti-inflammation. Agarose plate meth? od was used to study the inhibitory effect of the water extract of Sappa Lignum (0.03g / ml, 0.06g / ml, 0.09 g / ml, 0.12g / ml, 0.15g / ml) on the hydrolysis activity of snake venom sPLA2. The diameter of transparent circle and pore were measured and the inhibition rate was calculated according to the stan? dard curve of enzyme activity. The results show that the water extract of 0.15g/ml has the inhibitory ac? tivity against the hydrolysis activity of sPLA2 from the venom of Agkistrodon acutus.

参考文献/References:

[1] Moreira V, de Castro Souto PCM, Ramirez Vinolo MA, et al. A catalytically- inactive snake venom Lys49 phospholipase A2 homolog induces expression of cyclooxygenase- 2 and pro duction of prostaglandins through selected signaling pathways in macrophages. European Journal of Pharmacology 2013, 708: 68-79. [2] Wei J-F, Wei X-l, Chen Q-Y, et al. Induction of inflammato ry cell accumulation by TM- N49 and promutoxin, two novel phospholipase A2. Toxicon 2010, 56:580-588. [3] Zuliani JP, Fernandes CM, Zamuner SR, et al. Inflammatory events induced by Lys- 49 and Asp- 49 phospholipases A2 isolated from Bothrops asper snake venom: role of catalytic activity. Toxicon 2005, 45:335-346. [4] Da Silva SL, Calgarotto AK, Chaar JS, et al. Isolation and characterization of ellagic acid derivatives isolated from Case aria sylvestris SW aqueous extract with anti- PLA(2) activity. Toxicon 2008, 52:655-666. [5] Flora Republicae Popularis Sinicae(中国植物志). Beijing:sci ence press,1986,39:105. [6] 王振月，王宗权，周亚滨，李春燕.苏木化学成分的研究 （Ⅰ）[J].天然产物研究与开发.2010，22:590-593. [7] 张琼，刘雪婷，梁敬钰.云实的化学成分[J].中国天然药物， 2008，6（3）:168-171. [8] 蔡晨秋.苏木的化学成分研究[D].北京：中央民族大学， 2012:56 [9] Garcia Denegri ME, Acosta OC, Huancahuire- Vega S, et al. Isolation and functional characterization of a new acidic PLA (2) Ba SpII RP4 of the Bothrops alternatus snake venom from Argentina. Toxicon 2010, 56:64-74. [10] 郭春风，周亚滨，陈会君，等．苏木乙酸乙酯提取物对慢 性柯萨奇病毒性心肌炎小鼠外周血中TNF-α 的影响［J］ .中医药学报，2014，42( 5) : 18-20． [11] WASHIYAMA M，SASAKI Y，HOSOKAWA T，et al． An ti- inflammatory constituents of Sappan Lignum.［J］． Biol Pharm Bull，2009，32( 5) : 941-944．

备注/Memo

备注/Memo:
*基金项目：衢州职业技术学院科研项目(QZYY1520) 作者简介：乐贞（1989-），女，汉族，江西上饶人，硕士，助教，研究方向：生物医药。
更新日期/Last Update: 2018-12-10