[1]陈桂华 李翔辉 郁川*.TAT-Apoptin基因原核表达载体的构建及其表达鉴定[J].现代农业研究,2019,(11):96-98.
　Chen Guihua Li Xianghui Yu Chuan *.Construction and expression Identification of prokaryotic expression Vector of TAT-Apoptin Gene[J].Modern Agricultural Research,2019,(11):96-98.
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TAT-Apoptin基因原核表达载体的构建及其表达鉴定
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《现代农业研究》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年11期
页码:
96-98
栏目:
生物科学
出版日期:
2019-11-04

文章信息/Info

Title:
Construction and expression Identification of prokaryotic expression Vector of TAT-Apoptin Gene
作者:
陈桂华 李翔辉 郁川*
1.漯河食品职业学院462300；2.河南科技大学动物疫病与公共安全重点实验室471003
Author(s):
Chen Guihua Li Xianghui Yu Chuan *
1.Luohe Food Vocational College 462300 2.Henan University Of Science and Technology 471003
关键词:
TAT-Apoptin 融合蛋白; 重组载体pET-32a-TAT-Apoptin; 分泌性表达
Keywords:
TAT-Apoptin fusion protein;  secretory expression of recombinant vector pET-32a-TATApoptin
摘要:
为得到TAT-Apoptin 融合蛋白，以pMD-19T-Apoptin 为模板PCR 扩增出TATApoptin 基因，构建重组载体pET-32a-TAT-Apoptin，并对TAT-Apoptin 蛋白进行表达。PCR 和测序表明，成功构建重组载体pET-32a-TAT-Apoptin，进一步对其进行蛋白表达，发现该蛋 白能与Apoptin 多抗发生特异性结合。以上结果证明，TAT-Apoptin 基因原核表达载体构建成 功，并能在Rosetta 中进行分泌性表达。
Abstract:
In order to obtain TAT-Apoptin fusion protein, TAT-Apoptin gene was amplified by PCR with pMD-19T-Apoptin as template, the recombinant vector pET-32a-TAT-Apoptin, was constructed and the TAT-Apoptin protein was expressed. PCR and sequencing showed that the recombinant vector pET-32a-TAT-Apoptin, was successfully constructed to further express the protein, and it was found that the protein could specifically bind to Apoptin polyantibodies. The above results show that TATapoptin base The prokaryotic expression vector was successfully constructed and secreted in Rosetta.

参考文献/References:

[1] Yuasa N, Taniguchi T, Yoshida I. Isolation and some proper ties of an agent iducing anemia in chicks[J]. Avian Dis, 1979, 23(2): 366-385. [2] Noteborn MH, Todd D, Verschueren CA, et al. A single chicken anemia virus protein induces apoptosis [J]. J Virol, 1994, 68(1): 346-351. [3] S Debaisieux, F Rayne, H Yezid, et al. The ins and outs of HIV-1 Tat[J]. Traffic, 2012, 13(3): 355-363. [4] L Guelen, H Paterson, J Gaken, et al. TAT- apoptin is effi ciently delivered and induces apoptosis in cancer cells[J]. Oncogene, 2004, 23(5): 1153-1165. [5] LT Jia, SY Chen, AG Yang. Cancer gene therapy targeting cellular apoptosis machinery[J]. Cancer Treat Rev, 2012, 38 (7): 868-876.

备注/Memo

备注/Memo:
作者简介：陈桂华(1987-)，女，汉族，河南漯河人，硕士研究生，助教，研究方向为动物疫病防控。 李翔辉（1989-），男，汉族，河南漯河人，硕士研究生，讲师，研究方向为预防兽医学。 通信作者：郁川（1985-），男，汉族，河南安阳人，博士，讲师，研究方向为预防兽医学。
更新日期/Last Update: 1900-01-01